TLS Online TPP Program

#Id: 2832

#Unit 13. Methods in Biology

Examples of group-specific ligands commonly used in affinity chromatography

TLS Online TPP Program

#Id: 2833

#Unit 13. Methods in Biology

The peptide or protein (known as a ‘ tag’) can be placed at the N- or C-terminal ends and provides a means for the fusion protein to be selectively purified from the cell extract by affinity chromatography.

TLS Online TPP Program

#Id: 2834

#Unit 13. Methods in Biology

Fusion Constructs to Aid Protein Purification can be created by using Glutathione- S -transferase (GST), Maltose-Binding Protein (MBP), Poly-Histidine Tag (His-tag), FLAG ®

TLS Online TPP Program

#Id: 2835

#Unit 13. Methods in Biology

GST tagged protein are separated by using Glutathione (a tripeptide with a g-peptide linkage, g -Glu-Cys-Gly), displacing the bound GST moiety.

TLS Online TPP Program

#Id: 2836

#Unit 13. Methods in Biology

Maltose-Binding Protein (MBP) is also used for fusion tag for protein which are extremely GST

TLS Online TPP Program

#Id: 2837

#Unit 13. Methods in Biology

Maltose-Binding Protein (MBP) One-step purification can be achieved using amylose resin