Both genetic and physical maps provide information about the relative positions of and distances between genes. Genetic maps are based on rates of recombination and are measured in recombination frequencies (centiMorgans or map units). 

TLS Online TPP Program

#Id: 1847

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 1848

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1849

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light 

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 1850

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1851

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1852

#Unit 3. Fundamental Processes

The CTD contains a series of repeats of the heptapeptide sequence: Tyr-Ser-Pro-Thr-Ser-Pro-Ser.