TLS Online TPP Program

#Id: 2962

#Unit 3. Fundamental Processes

When replication is initiated, Cdc6, and Cdt1 are displaced. The degradation of Cdc6 prevents reinitiation.

TLS Online TPP Program

#Id: 2963

#Unit 3. Fundamental Processes

The striking feature is that ORC remains bound at the origin through the entire cell Cycle.

TLS Online TPP Program

#Id: 2964

#Unit 3. Fundamental Processes

The major role of ORC is to identify the origin to the Cdc6 and MCM proteins that control initiation and licensing.

TLS Online TPP Program

#Id: 2965

#Unit 3. Fundamental Processes

In yeast, Cdc6 is a highly unstable protein, with a half-life of <5 minutes. It is synthesized during G1 and typically binds to ORC between the exit from mitosis and late G1.

TLS Online TPP Program

#Id: 2966

#Unit 3. Fundamental Processes

pathway. Cdt1  is initially stabilized by the protein Geminin, which prevents its degradation, and subsequent Geminin binding prevents its reuse.

TLS Online TPP Program

#Id: 2967

#Unit 3. Fundamental Processes

In yeast, the replication helicase MCM2-7 (minichromosome maintenance) complex enters the nucleus as an inactive double hexamer during mitosis.