Most mRNAs decay stochastically (like the decay of radioactive isotopes), and as a result mRNA stability is usually expressed as a half- life (t½).

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#Id: 2540

#Unit 13. Methods in Biology

In PCR, the primer length influences the rate at which primer molecules pair with template DNA; this rate decreases as the primer length increases.

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#Id: 2541

#Unit 13. Methods in Biology

The PCR primers must not have self complementary regions as this would lead to hairpin formation within primer molecules and make then unsuitable for PCR.

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#Id: 2542

#Unit 13. Methods in Biology

The partially hybridized primers may become extended by the DNA polymerase and are amplified; sometimes this may monopolize the PCR .

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#Id: 2543

#Unit 13. Methods in Biology

The two primers used in a PCR reaction have identical Tm for successful and specific amplification. 

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#Id: 2544

#Unit 13. Methods in Biology

The efficiency of amplification declines with an increase in the length of the target sequence.

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#Id: 2545

#Unit 13. Methods in Biology

Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.