Nurturing Life Sciences
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#Unit 2. Cellular Organization
Mnase digestion result on centrifugation and electrophoresis
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Core DNA has an invariant length of 146 bp the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases.
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Core DNA invariant length of 146 bp contains core and linker histones so Mnase cuts after H1 binding site.
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The nucleosome is a disc with DNA organized into 12/3 turns around the surface.
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The nucleosome consists of approximately equal masses of DNA and histones (including H1). The predicted mass of the nucleosome is 262 kD.