The bacterium Ralstonia eutropha has been used as a model organism for the commercial production of PHAs.

TLS Online TPP Program

#Id: 8218

#Unit 2. Cellular Organization

Sorting signal that directs lysosomal hydrolases from the TGN to the late endosome is- Mannose 6-phosphate (M6P).

TLS Online TPP Program

#Id: 8219

#Unit 2. Cellular Organization

M6P residues are generated in the cis-Golgi by two Golgi-resident enzymes. 

1. N-acetylglucosamine (GlcNAc) phosphotransferase
2. Phosphodiesterase

TLS Online TPP Program

#Id: 8221

#Unit 2. Cellular Organization

The M6P receptor protein binds to M6P at pH 6.5–6.7 in the TGN lumen and releases it at pH 6, in the lumen of endosomes.

TLS Online TPP Program

#Id: 8223

#Unit 2. Cellular Organization

Retromer

a coat protein complex specialized for endosome-to-TGN transport, recycle the M6P receptor to the TGN

TLS Online TPP Program

#Id: 8224

#Unit 2. Cellular Organization

Lysosomal storage diseases:

• Hurler’s disease

• Inclusion-cell disease

TLS Online TPP Program

#Id: 8225

#Unit 2. Cellular Organization

Hurler’s disease- mutation in a structural gene that codes for an individual lysosomal hydrolase.