In the absence of Cerberus, Nodal is not activated and thus Pitx2 is not expressed

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#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

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#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

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#Unit 3. Fundamental Processes

Rpo I =14 subunits

Rpo II = 12 subunits

Rpo III = 17 subunits

There are five common subunits

Rpb5, Rpb6, Rpb8, Rpb10, Rpb12

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#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

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#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

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#Id: 1847

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.