Nurturing Life Sciences
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#Unit 13. Methods in Biology
In PCR, the length of primers is of great importance in ensuring that only the target sequence is amplified.
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In PCR, the primer length influences the rate at which primer molecules pair with template DNA; this rate decreases as the primer length increases.
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The PCR primers must not have self complementary regions as this would lead to hairpin formation within primer molecules and make then unsuitable for PCR.
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The partially hybridized primers may become extended by the DNA polymerase and are amplified; sometimes this may monopolize the PCR .
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The two primers used in a PCR reaction have identical Tm for successful and specific amplification.
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The efficiency of amplification declines with an increase in the length of the target sequence.
