DNA polymerase “grips” the template and the incoming nucleotide when a correct base pair is made via Arg, Lys and Tyr residue in its alpha helix

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#Id: 893

#Unit 13. Methods in Biology

RE’ has two functions-

a) Restriction of foreign genome

b) Modification/methylation of host genome using SAM

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#Id: 894

#Unit 13. Methods in Biology

Recognize the same sequence and cut at exactly the same position, these enzymes are called isoschizomers.

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#Id: 895

#Unit 13. Methods in Biology

Isoschizomers are applicable in recognizing methylation sites genomes.

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#Id: 896

#Unit 13. Methods in Biology

Two REs recognize the same DNA sequence motif but cleave at different positions, then they are called neoschizomers

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#Id: 897

#Unit 13. Methods in Biology

Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation

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#Id: 898

#Unit 13. Methods in Biology

Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.