In the absence of gibberellin, the DELLA proteins positively regulate GID1, GA20ox, and GA3ox (plus signs), and negatively regulate GA2ox (minus sign). Conversely, both bioactive gibberellin and the GID1 receptor enhance DELLA repressor degradation (plus signs), while GA2ox blocks DELLA repressor degradation (minus signs).

TLS Online TPP Program

#Id: 2926

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2930

#Unit 3. Fundamental Processes

The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

TLS Online TPP Program

#Id: 2931

#Unit 3. Fundamental Processes

Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

TLS Online TPP Program

#Id: 2932

#Unit 3. Fundamental Processes

Replication in bacteria and in eukaryotes is licensed and permitted to occur only once per cell cycle.

TLS Online TPP Program

#Id: 2933

#Unit 3. Fundamental Processes

The E. coli oriC is fully methylated at the beginning of replication.

TLS Online TPP Program

#Id: 2934

#Unit 3. Fundamental Processes

oriC is hemimethylated and remains in that condition for approximately 13 minutes.