Nurturing Life Sciences
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#Unit 13. Methods in Biology
Recognize the same sequence and cut at exactly the same position, these enzymes are called isoschizomers.
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Isoschizomers are applicable in recognizing methylation sites genomes.
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Two REs recognize the same DNA sequence motif but cleave at different positions, then they are called neoschizomers
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Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation
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Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.
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T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.