Rho factor is an essential protein in E. coli that causes transcription termination, accounts for almost half of E. coli terminators.

TLS Online TPP Program

#Id: 442

#Unit 2. Cellular Organization

The nucleosome have 200 bp of DNA with octameric basic proteins into a beadlike structure.

TLS Online TPP Program

#Id: 443

#Unit 2. Cellular Organization

Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA at linker DNA.

TLS Online TPP Program

#Id: 444

#Unit 2. Cellular Organization

The high-mobility group (HMG) are nonhistones and may contain Transcriptional Factors.

TLS Online TPP Program

#Id: 445

#Unit 2. Cellular Organization

Core DNA has an invariant length of 146 bp, the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases- DNA FINGER PRINTING

TLS Online TPP Program

#Id: 446

#Unit 2. Cellular Organization

Linker DNA comprises the rest of the repeating unit. Its length varies from as little as 8 bp to as many as 114 bp per nucleosome.

TLS Online TPP Program

#Id: 447

#Unit 2. Cellular Organization

The predicted mass of the nucleosome is 262 kD.