Nurturing Life Sciences
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#Unit 3. Fundamental Processes
HNO2 or Nitrous Acid is deaminating agent causes removal of –NH2 for nitrogenous bases.
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Adenine is deaminated to hypoxanthine.
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Cytosine is deaminated to Uracil.
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Guanine is deaminated to xanthine.
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Nitrous acid induces transitions in both directions, A:T ↔ G:C.
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The +vely charged acridine dyes or acridine orange are potent mutagens that induce frameshift mutations by Intercalation.