Nurturing Life Sciences
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#Unit 13. Methods in Biology
Principle of purification of an enzyme by affinity chromatography.
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Examples of group-specific ligands commonly used in affinity chromatography
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GST tagged protein are separated by using Glutathione (a tripeptide with a g-peptide linkage, g -Glu-Cys-Gly), displacing the bound GST moiety.
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