#Id: 6777
#Unit 3. Fundamental Processes
Mutation of either the 5′ or the 3′ invariant splice-site bases at the ends of the intron resulted in pre-mRNAs that were bound by snRNPs to form spliceosomes; however, RNA splicing was blocked, and the pre-mRNA was retained in the nucleus.
In contrast, mutation of both the 5′ and 3′ splice sites in the same pre-mRNA resulted in export of the unspliced pre-mRNA, although less efficiently than for the spliced mRNA, probably because of the absence of an exon-junction complex. When both splice sites were mutated, the pre-mRNAs were not efficiently bound by snRNPs, and consequently, their
export was not blocked.