Nurturing Life Sciences
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#Unit 3. Fundamental Processes
The bacterial DnaA protein is the replication initiator; it binds sequence specifically to multiple sites (dnaA boxes) in oriC.
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Activity of the replication origin is controlled is DNA methylation
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The E. coli oriC contains 11 copies of the GATC sequence
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GATC is a target for methylation at the N6 position of A by Dam Methylase enzyme which uses SAM as methyl group donor
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Several of methylation sites overlap dnaA boxes
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DnaA is an ATP-binding protein and its binding to DNA is affected depending on whether ATP