TLS Online TPP Program

#Id: 2599

#Unit 13. Methods in Biology

TaqMan assay also applicable in analyzing variations in the allele or deletion 

of base substitution.

TLS Online TPP Program

#Id: 2600

#Unit 13. Methods in Biology

Touch down PCR are derived to enhance primer binding in the annealing step.

TLS Online TPP Program

#Id: 2601

#Unit 13. Methods in Biology

A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .

TLS Online TPP Program

#Id: 2602

#Unit 13. Methods in Biology

Long primers forms most probably dimers, so taq pol amplifying this (in low temp) also.

TLS Online TPP Program

#Id: 2603

#Unit 13. Methods in Biology

The SNP mutation responsible for sickle-cell disease changes amino acid 6 in haemoglobin β from glutamic acid (E) to valine (V). 

TLS Online TPP Program

#Id: 2604

#Unit 13. Methods in Biology

The sequence of the PCR product from a heterozygous carrier shows both the normal (HbβA) and mutant (HbβS) nucleotides at the position of the substitution SSR genotyping by PCR product electrophoresis.