TLS Online TPP Program

#Id: 2467

#Unit 13. Methods in Biology

Slab gels can develop a “Smiling Effect” When in a gel more Joule heat is produced than can be dissipated, leads to the higher mobility of the ions in the center, resulting in an uneven distribution of the zones, These zones bend up at the two lateral sides, causing “smiling.” 

TLS Online TPP Program

#Id: 2468

#Unit 13. Methods in Biology

Phenomenon of Smiling Effect is largely applicable in Gel Electrophoresis &  PAGE

TLS Online TPP Program

#Id: 2469

#Unit 13. Methods in Biology

Phenomenon of Electroendosmosis is largely applicable in Free Solution & Capillary.

TLS Online TPP Program

#Id: 2470

#Unit 13. Methods in Biology

Electrophoretic mobility and Electroendosmosis force combinedly functions in Capillary zone Electrophoresis

TLS Online TPP Program

#Id: 2471

#Unit 13. Methods in Biology

In electrophoretic separation the phenomenon of electroendosmosis (also known as electro-osmotic flow) which is due to the presence of charged groups on the surface of the support medium.

TLS Online TPP Program

#Id: 2472

#Unit 13. Methods in Biology

Three basically different electrophoretic separation methods are performed in practice nowadays

 Electrophoresis, sometimes called Zone Electrophoresis (ZE) GE

 Isotachophoresis (ITP) SDS PAGE

Isoelectric focusing (IEF).