In nonselective autophagy, a bulk portion of cytoplasm is sequestered in autophagosomes

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#Id: 909

#Unit 13. Methods in Biology

Tetrazolium compound MTT (3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide MTT Assay is calorimetric assay to detect cell division

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#Id: 910

#Unit 13. Methods in Biology

Diphenyltetrazolium bromide, a yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm

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#Id: 911

#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time.

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#Id: 912

#Unit 13. Methods in Biology

The MTT assay can measure cell proliferation or cell death.

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#Id: 913

#Unit 13. Methods in Biology

bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells.

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#Id: 914

#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death.