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TLS Online TPP Program

#Id: 8279

Charge–charge (ionic) interactions
These interactions occur between the side chains of oppositely charged residues as well as between the NH3+ and COO− groups at the ends of polypeptide.

#Unit 1. Molecules and their Interaction Relevant to Biology #Stablizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction) #Part B Pointers
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TLS Online TPP Program

#Id: 2829

#Unit 13. Methods in Biology

Affinity chromatography exploits the unique property of extremely specific biological interactions to achieve separation and purification.
TLS Online TPP Program

#Id: 2830

#Unit 13. Methods in Biology

Affinity chromatography used for the purification of enzymes, nucleotides, nucleic acids, immunoglobulins, membrane receptors and even to whole cells and cell fragments.
TLS Online TPP Program

#Id: 2831

#Unit 13. Methods in Biology

Principle of purification of an enzyme by affinity chromatography.
TLS Online TPP Program

#Id: 2832

#Unit 13. Methods in Biology

Examples of group-specific ligands commonly used in affinity chromatography
TLS Online TPP Program

#Id: 2833

#Unit 13. Methods in Biology

The peptide or protein (known as a ‘ tag’) can be placed at the N- or C-terminal ends and provides a means for the fusion protein to be selectively purified from the cell extract by affinity chromatography.
TLS Online TPP Program

#Id: 2834

#Unit 13. Methods in Biology

Fusion Constructs to Aid Protein Purification can be created by using Glutathione- S -transferase (GST), Maltose-Binding Protein (MBP), Poly-Histidine Tag (His-tag), FLAG ®