Ca2+ dependence of vertebrate skeletal muscle contraction, depends on :

action potential

specialized accessory proteins Tropomyosin,

Troponin (T, I, and C)

TLS Online TPP Program

#Id: 907

#Unit 13. Methods in Biology

Flow cytometry is majorly used with fluorescence dyes and attached antibodies for cells and dyes.

TLS Online TPP Program

#Id: 908

#Unit 13. Methods in Biology

Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting. 

TLS Online TPP Program

#Id: 909

#Unit 13. Methods in Biology

Tetrazolium compound MTT (3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide MTT Assay is calorimetric assay to detect cell division

TLS Online TPP Program

#Id: 910

#Unit 13. Methods in Biology

Diphenyltetrazolium bromide, a yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm

TLS Online TPP Program

#Id: 911

#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time.

TLS Online TPP Program

#Id: 912

#Unit 13. Methods in Biology

The MTT assay can measure cell proliferation or cell death.