Domain

Domains are formed by combinations of SS, SSS, motifs and connected by loops and turns.

Highly conversed and may be common in most unrelated proteins

They evolve

Can function Independently

Average 100 amino acids 

Single hydrophobic core

TLS Online TPP Program

#Id: 875

#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

TLS Online TPP Program

#Id: 876

#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å

TLS Online TPP Program

#Id: 877

#Unit 13. Methods in Biology

GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.

TLS Online TPP Program

#Id: 878

#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

TLS Online TPP Program

#Id: 879

#Unit 13. Methods in Biology

Short tandem repeats (STRs) are short repeated sequences of DNA (1–10 bp) that account for approximately  3% of the human genome.

TLS Online TPP Program

#Id: 880

#Unit 13. Methods in Biology

AAAAAAAAAAAAAAA (a 1-base repeat) and CACACACACACACACACACACA (a 2-basen repeating), CAGCAGCAGCAGCAGCAG (a 3 base repeating) are most common.