diisopropylphosphofluoridate, reacts with a serine OH group in the active site of esterases such as acetylcholinesterase/chymotrypsin, whilst the organomercury compound p-hydroxymercuribenzoate reacts with a cysteine group, in both cases resulting in covalent bond formation and enzyme inhibition.

TLS Online TPP Program

#Id: 4759

#Unit 5. Developmental Biology

PAL-1 activity is needed for the normal development of the somatic (but not the germline) descendants of the P2 blastomere, specifies muscle production.

TLS Online TPP Program

#Id: 4760

#Unit 5. Developmental Biology

Embryos lacking PAL-1 have no somatic cell types derived from the C and D stem cells.

TLS Online TPP Program

#Id: 4761

#Unit 5. Developmental Biology

PAL-1 is regulated by the MEX-3 protein, an RNA binding protein that inhibit the translation of pal-1 mRNA.

TLS Online TPP Program

#Id: 4762

#Unit 5. Developmental Biology

In mex-3-deficient mutants, PAL-1 is seen in every blastomere.

TLS Online TPP Program

#Id: 4763

#Unit 5. Developmental Biology

SKN-1 also inhibits PAL-1 (preventing it from becoming active in the EMS cell).

TLS Online TPP Program

#Id: 4764

#Unit 5. Developmental Biology

In the germ line, PAL-1 synthesis is prevented by the PUF-8 protein, which binds to the 3′ UTR of pal-1 mRNA and blocks its translation.