Nurturing Life Sciences
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#Unit 13. Methods in Biology
The time taken for each cycle is considerably longer than 3 min (0.5 + 1.5 +1 min)
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Thermal modulations are used in PCR for the functions of helicase and polymerase.
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In the PCR denaturation temperature calculated by using the Wallace rule, which is, Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs).
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The size of a PCR product is mainly determined by the binding location of the PCR primers.
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