The size of a PCR product is mainly determined by the binding location of the PCR primers.

TLS Online TPP Program

#Id: 2747

#Unit 13. Methods in Biology

RAPDs (Random amplified polymorphic DNAs) are obtained by using a PCR (polymerase chain reaction) equipment or a thermal cycler. 

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#Id: 2748

#Unit 13. Methods in Biology

The RAPD technique is based on PCR, which uses a DNA polymerase that is resistant to the high temperature needed for DNA denaturation; this permits the enzyme to be used repeatedly during the amplification process. 

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#Id: 2749

#Unit 13. Methods in Biology

RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible. 

TLS Online TPP Program

#Id: 2750

#Unit 13. Methods in Biology

Amplified Fragment length Polymorphism (AFLP) shares some features of both RFLP and RAPD analyses.

TLS Online TPP Program

#Id: 2751

#Unit 13. Methods in Biology

AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

TLS Online TPP Program

#Id: 2752

#Unit 13. Methods in Biology

AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.