Size of the primer negatively affects the efficiency of PCR.

TLS Online TPP Program

#Id: 377

#Unit 2. Cellular Organization

For negatively supercoiled circle W < 0 

For a positively coiled superhelix W > 0.

 

TLS Online TPP Program

#Id: 378

#Unit 2. Cellular Organization

Topological states of covalently closed, circular (ccc) DNA. 

TLS Online TPP Program

#Id: 379

#Unit 2. Cellular Organization

A conserved region found in every core histone, called the histone-fold domain HFD.

TLS Online TPP Program

#Id: 380

#Unit 2. Cellular Organization

The histone-fold domain is composed of three a-helical regions separated by two short unstructured loops.

 

TLS Online TPP Program

#Id: 381

#Unit 2. Cellular Organization

Histone-fold domain mediates the assembly of Histone-only Intermediates

TLS Online TPP Program

#Id: 382

#Unit 2. Cellular Organization

H3 and H4 histones first form heterodimers that then come together to form a tetramer with two molecules each of H3 and H4. While H2A and H2B form heterodimers in solution but not tetramers. This assembly without DNA called Histone-only Intermediates