TLS Online TPP Program

#Id: 2874

#Unit 13. Methods in Biology

The Beer–Lambert law is valid for low concentrations only.

TLS Online TPP Program

#Id: 2875

#Unit 13. Methods in Biology

According to the Beer–Lambert law, absorbance is linearly proportional to the concentration of chromophores.

TLS Online TPP Program

#Id: 2876

#Unit 13. Methods in Biology

Beer–Lambert law might not be the case any more in samples with high absorbance.

TLS Online TPP Program

#Id: 2877

#Unit 13. Methods in Biology

Molecular events might lead to deviations from the Beer–Lambert law. For instance, chromophores might dimerise at high concentrations and, as a result, might possess different spectroscopic parameters.

TLS Online TPP Program

#Id: 2878

#Unit 13. Methods in Biology

Bacterial cultures do not absorb the incoming light. Instead, the light is scattered and thus, the spectrometer will record an apparent absorbance (sometimes also called attenuance). In this case, the observed parameter is called optical density (OD).

TLS Online TPP Program

#Id: 2879

#Unit 13. Methods in Biology

Instruments specifically designed to measure turbid samples are nephelometers or Klett meters.