The Hydrophobic Effect Has the Greatest Influence on Protein Stability. The hydrophobic effect, which causes nonpolar substances to minimize their contacts with water, is the major determinant of native protein structure.

TLS Online TPP Program

#Id: 1862

#Unit 3. Fundamental Processes

RNA Polymerase I has a Bipartite Promoter

 

TLS Online TPP Program

#Id: 1981

#Unit 3. Fundamental Processes

This structure is called a cap.

TLS Online TPP Program

#Id: 1982

#Unit 3. Fundamental Processes

After transcription initiation, Pol II is paused 30 nucleotides downstream from the initiation site, recruits of the capping enzymes.

TLS Online TPP Program

#Id: 1983

#Unit 3. Fundamental Processes

Capping is done by a dimeric nuclear enzyme which has two subunits and three functions

1. Guanylyl-transferase (GT)

2. Methyltransferase (MT)

TLS Online TPP Program

#Id: 1984

#Unit 3. Fundamental Processes

Structure of cap is

   

TLS Online TPP Program

#Id: 1985

#Unit 3. Fundamental Processes

Binding of the capping enzyme to the TFIIH mediated serine 5–phosphorylated CTD stimulates the activity of the enzyme.