Nurturing Life Sciences
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#Unit 13. Methods in Biology
HOT START PCR were devised to prevent non – specific amplification at low temperature.
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FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å
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GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.
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The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.
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