Net primary productivity is commonly measured by harvesting a defined area of an ecosystem at the end of the growing season and determining the accumulated biomass of producers

TLS Online TPP Program

#Id: 4262

#Unit 12. Applied Biology

Characteristic Growth Pattern of Cultured Cells

TLS Online TPP Program

#Id: 4267

#Unit 12. Applied Biology

Trypsin is a serine protease that digest the extracellular protein or matrix protein so that cells get free.

TLS Online TPP Program

#Id: 4272

#Unit 12. Applied Biology

Dissociation of Adherent Cells

TLS Online TPP Program

#Id: 4274

#Unit 12. Applied Biology

To check the cell viability in cell suspension, trypan blue dye is used. This dye is excluded by viable cells but taken up by dead cells (nonviable cells).

TLS Online TPP Program

#Id: 4279

#Unit 12. Applied Biology

Cell viability is calculated as the number of viable cells divided by the total number of cells.

TLS Online TPP Program

#Id: 4281

#Unit 12. Applied Biology

The best method for cryopreserving cultured cells is storing them in liquid nitrogen in complete medium in the presence of a cryoprotective agent such as dimethylsulfoxide (DMSO) or glycerol