#Id: 2607

#Unit 13. Methods in Biology

SSR alleles differ in length.

Left and right primers correspond to unique sequences that flank the SSR locus.

PCR-amplification of two alleles of an SSR locus.

Gel electrophoresis distinguishes the alleles from each other. 

#Id: 2608

#Unit 13. Methods in Biology

Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. 

#Id: 2609

#Unit 13. Methods in Biology

RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

#Id: 2610

#Unit 13. Methods in Biology

RACE is sometimes called one-sided PCR or anchored PCR. 

#Id: 2611

#Unit 13. Methods in Biology

RACE can be used to amplify unknown 5' (5'-RACE) or 3' (3'-RACE) parts of RNA molecules where part of the RNA sequence is known and targeted by a GSP gene-specific primer.

#Id: 2612

#Unit 13. Methods in Biology

Our on-going research indicates that RLMRACE, PPM-RACE, and qRT-PCR are very effective in the verification of sequences of miRNA targets obtained by Degradome sequencing.