8 μM CO2 (0.038%) and 250 μM O2 (21%) at 25°C. Therefore, a specificity factor of 100 gives a Vc/Vo ratio of 3.2.

TLS Online TPP Program

#Id: 3670

#Unit 3. Fundamental Processes

mRNAs containing multiple ORFs are known as polycistronic mRNAs, and those encoding a single ORF are known as monocistronic mRNAs.

TLS Online TPP Program

#Id: 3671

#Unit 3. Fundamental Processes

A polycistronic prokaryotic message with three ORFs. Each ribosome binding site is indicated by a purple box labeled RBS.

TLS Online TPP Program

#Id: 3672

#Unit 3. Fundamental Processes

A monocistronic eukaryotic message. The 5’cap is indicated by a “ball” at the end of the mRNA.

TLS Online TPP Program

#Id: 3673

#Unit 3. Fundamental Processes

Prokaryotic mRNAs Have a Ribosome-Binding Site That Recruits the translational Machinery For translation to occur, the ribosome must be recruited to the mRNA.

TLS Online TPP Program

#Id: 3674

#Unit 3. Fundamental Processes

Many prokaryotic ORFs contain a short sequence upstream (on the 5’side) of the start codon called the ribosome binding site (RBS). This element is also referred to as a Shine –Dalgarno sequence.

TLS Online TPP Program

#Id: 3675

#Unit 3. Fundamental Processes

The RBS, typically located 3–9 bp on the 5’side of the start codon, is complementary to a sequence located near the 3’end of one of the ribosomal RNA components, the 16S ribosomal RNA (rRNA).