Nurturing Life Sciences
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#Unit 3. Fundamental Processes
Hydrolytic editing, is the polymerase backtracks by one or more nucleotides and cleaves the RNA product, removing the error-containing sequence.
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Hydrolytic editing is stimulated by Gre factors, which both enhance hydrolytic editing function and serve as elongation stimulating factors.
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The Nus proteins—joins polymerase in the elongation phase and promotes.
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Both polymerase removal and repair enzyme recruitment are performed by a single protein called TRCF.
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TRCF has an ATPase activity.
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TRCF binds to double-stranded DNA upstream of the polymerase and uses the ATPase motor to translocate along the DNA until it encounters the stalled RNA polymerase.