Nurturing Life Sciences
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#Unit 3. Fundamental Processes
Promoters with sequences closer to the consensus are generally “stronger” than those that match less well
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consensus sequence of TATAAT in which the leading TA and the final T are highly conserved. Upstream sequences around position –35 also have a region of sequence similarity, TTGACA.
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Sigma factor reduces the affinity for sequence independent binding and confers specificity for promoter.
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Sigma seems to stimulate both initiation and elongation.
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