Lack the form of sex based on meiosis that is familiar from animals and plants, they exchange genes using a variety of mechanisms that together are called “parasexuality”

TLS Online TPP Program

#Id: 4652

#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS. 

TLS Online TPP Program

#Id: 4653

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

TLS Online TPP Program

#Id: 4654

#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

Apoptotic cells

G1/G0

S

G2/M  

TLS Online TPP Program

#Id: 4655

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

TLS Online TPP Program

#Id: 4656

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 4657

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles