Bacteria frequently deploy a “quorum‐sensing” mechanism to facilitate successful colonization of plant tissue

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#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS. 

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#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

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#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

Apoptotic cells

G1/G0

S

G2/M  

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#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

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#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

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#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles