Quorum sensing in Erwinia carotovora is mediated by N‐acylhomoserine lactones, small molecules released from the bacterial cells into the surrounding medium.

TLS Online TPP Program

#Id: 3002

#Unit 13. Methods in Biology

Sodium bromide and sodium iodide are employed for the fractionation of lipoproteins
and the banding of DNA or RNA molecules, respectively.

TLS Online TPP Program

#Id: 3003

#Unit 13. Methods in Biology

For the separation of membrane vesicles derived from tissue homogenates, ultra-pure
DNase-, RNase and protease-free sucrose represents a suitable and widely employed
medium for the preparation of stable gradients.

TLS Online TPP Program

#Id: 3004

#Unit 13. Methods in Biology

For isopycnic separation , density centrifugation is continued until the buoyant density
of the particle of interest and the density of the gradient are equal.

TLS Online TPP Program

#Id: 4588

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 4589

#Unit 13. Methods in Biology

 Flow cytometry is based on light scattering and fluorescence 

TLS Online TPP Program

#Id: 4590

#Unit 13. Methods in Biology

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell