Nurturing Life Sciences
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#Unit 13. Methods in Biology
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Electron microscopy under optimal conditions provides, a resolution of 0.10 nm wavelength.
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Electromagnetic lenses are used to focus a high-velocity electron beam instead of the visible light.
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Thicker sections must be fixed, dehydrated, embedded in plastic, sectioned, and then stained with electron dense heavy metals for TEM.
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For TEM two types of staining is done:
Negative staining
Metal shadowing