Nurturing Life Sciences
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#Id: 707
#Unit 3. Fundamental Processes
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seqA is part of a negative regulatory circuit that prevents origins from being remethylated.
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A locus called dat has a large concentration of DnaA-binding sites. It binds about 80 more DnaA molecules than the origin.
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Deletion of dat causes initiation to occur more frequently. This significantly increases the amount of DnaA available to the origin
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DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Hda.
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Hda is recruited by DNA Polymerase in previous cycle and it acts to switch off DnaA, preventing a second round of replication.