Nurturing Life Sciences
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#Unit 13. Methods in Biology
Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes
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Bromodeoxyuridine-Based Assays for Cell Division Use Antibodies to Detect Newly Synthesized DNA
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Fluorescent probes are commonly used to detect rapid biochemical changes in single living cells
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Fluorescent probes are derived from a naturally occurring fluorescent protein, the green fluorescent protein (GFP) of the jellyfish Aequorea Victoria.
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When excited by absorption of a photon of light, GFP emits a photon (that is, it fluoresces) in the green region of the spectrum.
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The light-absorbing/ emitting center of GFP (its chromophore) comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67