In the absence of Wnts,

“free” β-catenin are targeted for degradation by a cytosolic destruction complex that is held together by the tumor suppressor protein Axin.

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#Unit 3. Fundamental Processes

Rpo I =14 subunits

Rpo II = 12 subunits

Rpo III = 17 subunits

There are five common subunits

Rpb5, Rpb6, Rpb8, Rpb10, Rpb12

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#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

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#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

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#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

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#Unit 3. Fundamental Processes

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#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light 

Alexa-568-labeled antibody- emit red light