TLS Online TPP Program

#Question id: 1042


A student complains that it makes no sense to worry about coliform bacteria in water, since we naturally possess harmless coliforms in our intestines anyway. Why do regulatory agencies worry about coliform bacteria in water supplies, then?

#Unit 4. Cell Communication and Cell Signaling
  1. The coliforms in our intestines can sometimes turn harmful, causing intestinal infections. We need to constantly be on guard against this happening. Coliforms in water might add to this potential problem.

  2. Not all coliforms are harmless and symbiotic with human beings. Some may carry genes/proteins that can make them dangerous to humans. It's best to keep coliforms OUT of our drinking water, since it's difficult to identify which ones might be harmless and which ones might be harmful.

  3. Regulatory agencies are worried that multiple types of coliforms in water might lead to greater antibiotic resistance in the bacterial populations. They try to keep them out of water to keep antibiotic resistance levels down.

  4. Regulatory agencies simply need something to do to justify their existence. There is no real danger from coliforms in water supplies.

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TLS Online TPP Program

#Question id: 635

#Unit 1. Molecules and their Interaction Relevant to Biology

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 107 M-1s-1 and the nonenzymatic rate constant (kn) is 3 x 10-16 s-1.  What is the value of the enzymeʹs catalytic proficiency?

TLS Online TPP Program

#Question id: 636

#Unit 1. Molecules and their Interaction Relevant to Biology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

Most

Least

(A)

Valine

Aspartate

(B)

Glycine

Arginine

(C)

Arginine

Glycine

(D)

Glutamate

Arginine

TLS Online TPP Program

#Question id: 637

#Unit 1. Molecules and their Interaction Relevant to Biology

Effect of temperature on enzymatic catalysis is analysed by the rate v of a chemical reaction varies with temperature according to the Arrhenius Equation which explains the sensitivity of enzyme reactions to temperature. A plot to determination of the activation energy Ea involves

TLS Online TPP Program

#Question id: 638

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the following class of enzymes with them

EnzymeClass of enzyme
A. Hexokinase1
B. Phosphoglucoisomerase2
C. Glyceraldehyde 3-phosphate dehydrogenase3
D. Phosphoglycerate mutase4
E. Enolase5
F. Aldolase

TLS Online TPP Program

#Question id: 639

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the coenzymes in column I enzymes to class of enzymes in column II

EnzymeReaction Type
A. Phosphofructokinase1. Redox reaction
B. Enolase2. Phosphoryl shift
C. Phosphoglycerate mutase3. Dehydration
D. Glyceraldehyde 3 Phosphate dehydrogenase4. Phosphoryl transfer

TLS Online TPP Program

#Question id: 640

#Unit 1. Molecules and their Interaction Relevant to Biology

The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately