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TLS Online TPP Program
#Question id: 18959
#Unit 13. Methods in Biology
Vectors used for construction of genomic libraries, and their capacity is given match them correctly
A- λ EMBL series I - 300kb - 1,400 kb
B - Cosmids II - 45 kb
C – BACs and YACs (high capacity vectors) III - 20kb
TLS Online TPP Program
#Question id: 18960
#Unit 13. Methods in Biology
cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program
#Question id: 18961
#Unit 13. Methods in Biology
When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called
TLS Online TPP Program
#Question id: 18962
#Unit 13. Methods in Biology
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,
2. Their large size makes the handling procedures during cloning tedious and cumbersome
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.
These three problems have been resolved by deleting
TLS Online TPP Program
#Question id: 18963
#Unit 13. Methods in Biology
The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop
TLS Online TPP Program
#Question id: 18964
#Unit 13. Methods in Biology
Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of