Many G protein-coupled receptors contain seven transmembrane α-helical domains. The amino end of the protein lies at the exterior of the plasma membrane. Loops of amino acids connect the helices either at the exterior surface or on the cytosolic surface of the membrane. The loop on the cytosolic side between helices 5 and 6 is usually substantially longer than the others. Where would you expect to find the carboxyl end of the protein?

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#Question id: 18774

#Unit 13. Methods in Biology

The above Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. In these plots, r equals moles of bound ligand/mole antibody and c is the concentration of free ligand, (Ka) is slope while intercept on x axis represent 

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#Question id: 18775

#Unit 13. Methods in Biology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

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#Question id: 18776

#Unit 13. Methods in Biology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 

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#Question id: 18777

#Unit 13. Methods in Biology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

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#Question id: 18778

#Unit 13. Methods in Biology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

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#Question id: 18779

#Unit 13. Methods in Biology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true 

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response. 

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests