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TLS Online TPP Program

#Question id: 1244

Which type of experimental evidence shows that the intrinsic GTPase activity of the Gα subunit is important for terminating effector activation?

#Unit 4. Cell Communication and Cell Signaling

  1. A nonhydrolyzable GTP analog that can bind to the Gα subunit but cannot be hydrolyzed by the intrinsic GTPase, thereby activating the effector protein longer upon ligand-induced activation of the receptor. 

  2. A nonhydrolyzable GTP analog causes displacement of GDP with the modified GTP resulting in continuous activation of the receptor because the bound GTP analog cannot be hydrolyzed to GDP.

  3. A dominant active (constant activity) GEF causes stimulation of the effector protein for longer upon ligand-induced activation of the receptor.

  4. A dominant negative (no activity) GEF causes stimulation of the effector protein for longer upon ligand-induced activation of the receptor.

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TLS Online TPP Program

#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II
Column I                                                                          Column II
1. Texas                                                                            a. far red
2. Fluorescein                                                                   b. red
3. Rhodamine                                                                   c. blue 
4. Cy5                                                                               d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?
TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?
TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?