Following statements are regarding to the phosphoinositide pathway.

A. many activated RTKs and cytokine receptors initiate phosphoinositide pathway by recruiting the enzyme phosphatidylinositol-3 kinase (PI-3 kinase) to the membrane.

B. PI-3 kinase adds a phosphate to the 3′ carbon in one of two separate phosphatidylinositol substrates, leading to formation of two phosphatidylinositol 3-phosphates: PI(3,4)P2 or I(3,4,5)P3

C. PI-3 kinase pathway can trigger cell division and prevent apoptosis, thus ensuring cell survival by the activation of PKB.

D. Activated PKB phosphorylates FOXO3a, which induces the expression of several antiapoptotic genes.

Which of the following combination is correct?

#Question id: 4402

#Unit 3. Fundamental Processes

Chromatin-mediated repression of transcription involves

a. modification of lysine residues in histones. 

b. large, multiprotein complexes.

c. acetylation of histone tails.   

d. deacetylation of histone tails.

#Question id: 4403

#Unit 3. Fundamental Processes

 Which of the following does (do) not require a DNA helicase activity?

a. SWI/SNF function  b. Pol II open-complex formation

c. transcription-factor binding to DNA

d. deacetylation of histone tails

#Question id: 4404

#Unit 3. Fundamental Processes

The yeast two-hybrid system can be used to identify

a. proteins that interact with a known or “bait” protein.

b. proteins that interact with a “fish” domain.

c. cDNAs that encode interacting proteins.

d. co-activators and co-repressors.

#Question id: 4405

#Unit 3. Fundamental Processes

Which of the following can occur when steroid hormone binds to its cognate receptor?

a. conformational changes in the receptor

b. translocation of the receptor out of the cell membrane

c. binding of the receptor to DNA response elements

d. stimulation of transcription

#Question id: 4406

#Unit 3. Fundamental Processes

An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?

#Question id: 4407

#Unit 3. Fundamental Processes

The general transcriptional factors are used by all sets of RNA Polymerase. Which of the following GTF is/are commonly used by all RNA Polymerases?

A. TBP

B. TFIID

C. TFIIIC

D.TFIIE

E. TFIIH