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TLS Online TPP Program
#Question id: 13057
#Unit 13. Methods in Biology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 15677
#Unit 2. Cellular Organization
Ultraviolet (UV) radiation around 260 nm is quite lethal but does not penetrate
1. glass
2. dirt films
3. Water
March the correct answer
TLS Online TPP Program
#Question id: 31314
#Unit 6. System Physiology – Plant
Pyruvate is precursor of which secondary metabolite biosynthetic pathway
TLS Online TPP Program
#Question id: 2944
#Unit 2. Cellular Organization
DNA replication at each origin occurs only once during the cell cycle because of:
TLS Online TPP Program
#Question id: 19895
#Unit 12. Applied Biology
In agriculture the biosensors are used to detect the __________________?