This member of the AKAP family, designated mAKAP, anchors both PDE and the regulatory subunit  of PKA to the nuclear membrane, maintaining them in a negative feedback loop that provides close local control of the ATP level and PKA activity.

B- The basal level of PDE activity in the presence of hormone (resting state) keeps cAMP levels below those necessary for PKA activation. 

C- Activation of β-adrenergic receptors causes an increase in cAMP to a level in excess of that which can be degraded by PDE. 

D- The resulting binding of cAMP to the R subunits of PKA releases the active catalytic (C) subunits into the cytosol. Some C subunits enter the nucleus, where they phosphorylate and thus activate certain transcription factors . Other C subunits phosphorylate PDE, stimulating its catalytic activity.

E-  Active PDE hydrolyzes cAMP, thereby driving cAMP levels back to basal levels and causing re-formation of the inactive PKA C-R complex. Subsequent de-phosphorylation of PDE returns the complex to the resting state.

TLS Online TPP Program

#Question id: 19885

#Unit 13. Methods in Biology

The plasmid used by Cohen and Boyer fr their transformation experiment was

TLS Online TPP Program

#Question id: 19886

#Unit 13. Methods in Biology

Gene cloning refers to the

I) Production of large number of copies of the gene being cloned

II) Production of asexual progeny from a single individual or a cell 

III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Unit 13. Methods in Biology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19889

#Unit 12. Applied Biology

The genetic monitoring and disease diagnosis are examples for ______________________ sensor?

TLS Online TPP Program

#Question id: 19890

#Unit 12. Applied Biology

QCM is a kind of a piezoelectric biosensor which detects mass change. What is the standard form of QCM biosensor ?