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TLS Online TPP Program
#Question id: 23167
#Unit 13. Methods in Biology
A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.
TLS Online TPP Program
#Question id: 23168
#Unit 13. Methods in Biology
What are the advantages of using secondary antibodies in indirect immunofluorescence?
1. It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program
#Question id: 23169
#Unit 13. Methods in Biology
Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled
TLS Online TPP Program
#Question id: 23170
#Unit 13. Methods in Biology
Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program
#Question id: 23171
#Unit 13. Methods in Biology
Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize
TLS Online TPP Program
#Question id: 23172
#Unit 13. Methods in Biology
Green fluorescent protein do contains a tripeptide sequence i.e. ser-tyr-gly but it only fluoresce when