9 In your study of a new bacterial species you have identified a transducing phage that you call Px. In addition you have worked out methods to make random transposon insertions into the bacterial genome. You have generated two different transposon insertion collections one with 105 random Tn5 (Kanr) insertions and the other with 105 random Tn10 (Tetr) insertions. You grow Px phage on the mixed collection of Tn5 insertions and use the resulting phage lysate to infect the mixed collection of Tn10 insertions. You select 10,000 Kanr  transductants and find that 80 of them are Tets. Use this information to estimate the total size of the bacterial genome assuming that both Tn5 and Tn10 insert randomly and that the average size of a fragment recombined into the recipient genome during Px transduction is 55 kbp. (Tn5 is about 5 kbp and Tn10 is 10 kbp.)

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#Question id: 15288

#Unit 5. Developmental Biology

Which of the following not a property of responder cell?

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#Question id: 15289

#Unit 5. Developmental Biology

Which of the following is a property of inducer cell?

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#Question id: 15290

#Unit 5. Developmental Biology

Lens formation requires sequential events whereby the anterior neural plate signals the anterior ectoderm to promote secretion of Pax 6, which renders the anterior ectoderm more receptive to secretions from the optic vesicle. The above can be best explained by which of the following phenomenon?

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#Question id: 15291

#Unit 5. Developmental Biology

Cell to cell communication is important in development of an organism. The ability of cells to respond to a specific inductive signal is called

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#Question id: 15296

#Unit 5. Developmental Biology

Match the following

Column I	Column II	Column III
A. Cytochalasin D	i. A sponge toxin	a. Enhances nucleation by binding and stabilizing actin dimers and thereby lowering the critical concentration.
B. Latrunculin	ii. A fungal alkaloid	b. Binds and sequesters G-actin, inhibiting it from adding to a filament end.
C. Jasplakinolide	iii. The “angel of death” mushroom	c. Binds at the interface between subunits in F-actin, locking adjacent subunits together and preventing actin filaments from depolymerizing.
D. Phalloidin		d. Depolymerizes actin filaments by binding to the (+) end of F-actin, where it blocksfurther addition of subunits.

Which one of the following is correct combination?

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#Question id: 15297

#Unit 5. Developmental Biology

 Which of the following statement is correct regarding mammalian cleavage?