9 In your study of a new bacterial species you have identified a transducing phage that you call Px. In addition you have worked out methods to make random transposon insertions into the bacterial genome. You have generated two different transposon insertion collections one with 105 random Tn5 (Kanr) insertions and the other with 105 random Tn10 (Tetr) insertions. You grow Px phage on the mixed collection of Tn5 insertions and use the resulting phage lysate to infect the mixed collection of Tn10 insertions. You select 10,000 Kanr  transductants and find that 80 of them are Tets. Use this information to estimate the total size of the bacterial genome assuming that both Tn5 and Tn10 insert randomly and that the average size of a fragment recombined into the recipient genome during Px transduction is 55 kbp. (Tn5 is about 5 kbp and Tn10 is 10 kbp.)

TLS Online TPP Program

#Question id: 27065

#Unit 1. Molecules and their Interaction Relevant to Biology

Determine R and S Configurations On A Fischer Projection given below 

TLS Online TPP Program

#Question id: 27066

#Unit 1. Molecules and their Interaction Relevant to Biology

Determine R and S Configurations On A Fischer Projection given below 

TLS Online TPP Program

#Question id: 27067

#Unit 1. Molecules and their Interaction Relevant to Biology

Choose the correct statement regarding the stereochemistry of amino acid

1. 16 amino acid except (Thr, Ile, Cys, Gly) L Fischer projection is equal to S and D is equal to R configuration 

2. Thr, Ile is special case because of two chiral center exist in L – Ile/Thr – 2S,3R and D-Ile/Thr–2R,3S configuration  

3. Cys exiest in L- Cys – R & D- Cys – S configuration 

4. Gly is optically inactive 

TLS Online TPP Program

#Question id: 27068

#Unit 1. Molecules and their Interaction Relevant to Biology

Isoleucine and Threonine have two chiral carbons so exists in multiple RS configuration, choose the correct option regarding the given fig.

1. a is D-threonine is (2S, 3S) threonine

2. b is L-threonine is (2R,3S) threonine

3. a is L-threonine is (2S, 3R) threonine

4. b is D-threonine is (2R,3S) threonine

TLS Online TPP Program

#Question id: 27069

#Unit 1. Molecules and their Interaction Relevant to Biology

Choose the correct option regarding the stereochemistry of given compound 

TLS Online TPP Program

#Question id: 27070

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following statements is correct regarding the stereochemistry of Thr, Ile 

1. Thr, Ile is special case because of two chiral center exist in L-Ile in 2R,3S and L-Thr in 2R,3S configuration 

2. Thr, Ile is special case because of two chiral center exist in D-Ile in 2R,3S and D-Thr in 2R,3S configuration 

3. Thr, Ile is special case because of two chiral center exist in L–Ile in 2S,3R and L–Thr in 2S,3R 

4. Thr, Ile is special case because of two chiral center exist in D–Ile in 2S,3R and D–Thr in 2S,3R